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KMID : 0921620110410030195
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Journal of Bacteriology and Virology
2011 Volume.41 No. 3 p.195 ~ p.204
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Proteomic Analysis of the Serum from Chicken Infected by Avian Influenza Virus
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Lee Hyung-Tae
Jung Kyoung-Hwa
Park Ji-Hyun
Ha Gun-Woo
Oh Youn-Kyoung
Oh Jin-Sik
Chai Young-Gyu
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Abstract
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Avian influenza (AI) is an infectious, low pathogenic virus that is endemic all over the world and poses a potential threat to the poultry industry. Vaccination is a widely used effective method to prevent avian influenza virus. Here we employed a comparative proteomics approach [two-dimensional electrophoresis (2-DE) and matrix assisted laser desorption ionization-time of flight (MALDI-TOF)] to characterize proteome in the sera from the specific pathogen free (SPF) chickens, the vaccinated chickens, and the naturally infected chickens. We identified total 58 proteins that were differentially expressed in the sera of three groups. Among them ovotransferrin and vitamin D-binding protein were more expressed in the sera of naturally infected chickens compare with other groups. Our results suggested that the level of these two proteins in the serum may help to discriminate the naturally infected chicken from the vaccinated chicken.
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KEYWORD
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Avian Influenza, H9 subtype, Two-dimensional electrophoresis, Chicken serum, MALDI-TOF
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